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If fresh tissue arrival is delayed, how should it be stored?

4C if delayed wrapped in gauze

Keeping fresh tissue when there’s a delay is all about slowing decay without freezing it. Refrigeration at about 4°C slows cellular enzymes and bacterial growth, helping preserve structural integrity for later processing. Wrapping the tissue in gauze helps keep it moist and protected while it sits in the fridge, so it doesn’t dry out or get contaminated. Other options don’t fit because room temperature in saline promotes rapid autolysis and microbial growth, body temperature (37°C) accelerates degradation, and freezing at -20°C can create ice-crystal artifacts that ruin histology and complicate sectioning.

-20C

RT in saline

37C in PBS

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